Journal: The Analyst

Article Title: Surface enhanced Raman scattering for the multiplexed detection of pathogenic microorganisms: towards point-of-use applications

doi: 10.1039/d1an00865j

Figure Lengend Snippet: A summary of the general approaches and studies discussed within this review

Article Snippet: Pazos-Perez et al. prepared a microorganism detection platform for the identification and quantification of the bacterial pathogens S . aureus , E . coli and Streptococcus agalactiae ( S . agalactiae ), on the millilitre scale and clinically relevant volumes of biofluids using antibody functionalised SERS-labelled silver NPs.

Techniques: Sampling, Sample Prep, Binding Assay

Journal: The Analyst

Article Title: Surface enhanced Raman scattering for the multiplexed detection of pathogenic microorganisms: towards point-of-use applications

doi: 10.1039/d1an00865j

Figure Lengend Snippet: Schematic illustrating the single-plex and multiplex detection assay. Assay format: (a) lectin (Con A) functionalised silver coated magnetic nanoparticles (Ag@MNPs) will bind to bacteria and the presence of the magnet will allow for magnetic separation of the bacteria from the sample matrix (b) SERS active silver nanoparticles (AgNPs) functionalised with a biorecognition molecule (antibody; Ab) and a unique SERS reporter are added. The mixture is shaken for 30 min before applying a magnet for a further 30 min and allowing the sample to collect. Any unbound matrix is gently removed, and the sample subsequently re-suspended in dH 2 O (c) The sample is then interrogated with a 532 nm laser beam and SERS signal obtained (green spectrum). When no target is present the functionalised AgNPs will be washed away, thus they will not bind to bacteria, so a minimum SERS signal obtained (red spectrum). (d) Multiplexing step: 3× AgNP conjugates each functionalised with a different Raman reporter and an antibody (which is specific for a bacterial pathogen) are added together with 3 bacterial pathogens and Con A (which binds to all three bacteria) functionalised Ag@MNPs. In the same way as the single-plex systems magnetic separation allows for the samples to be concentrated and analysed via a 532 nm laser. A SERS spectrum is obtained which contains characteristic peaks from the three Raman reporters and thus can be used to confirm if the targets are present. The image is reprinted from Kearns et al. , Copyright (2017), with permission from American Chemical Society.

Article Snippet: Pazos-Perez et al. prepared a microorganism detection platform for the identification and quantification of the bacterial pathogens S . aureus , E . coli and Streptococcus agalactiae ( S . agalactiae ), on the millilitre scale and clinically relevant volumes of biofluids using antibody functionalised SERS-labelled silver NPs.

Techniques: Multiplex Assay, Detection Assay, Bacteria, Multiplexing

Journal: ACS Omega

Article Title: Novel Size-Tunable and Straightforward Ultra-Small Nanoparticle Synthesis in a Varying Concentration Range of Glycerol as a Green Reducing Solvent

doi: 10.1021/acsomega.3c02697

Figure Lengend Snippet: UV–vis absorption spectrum of synthesized silver NPs (left) and graphical illustration at the absorption maxima to observe linearity (right) in varying concentrations of glycerol (10–100%).

Article Snippet: The TEM samples were made ready by drop-casting 2 μL of individual silver NPs in glycerol solution onto a Formvar–carbon-coated 400 mesh copper TEM grid (Agar Scientific, UK) at RT until being dried and imaged on a FEI Tecnai TF20 FEG high-resolution TEM system, operating at 200 kV.

Techniques: Synthesized

Journal: ACS Omega

Article Title: Novel Size-Tunable and Straightforward Ultra-Small Nanoparticle Synthesis in a Varying Concentration Range of Glycerol as a Green Reducing Solvent

doi: 10.1021/acsomega.3c02697

Figure Lengend Snippet: Size distribution analysis of silver NPs (10–100%) with DLS and (a) linear curve obtained from particle size data (b).

Article Snippet: The TEM samples were made ready by drop-casting 2 μL of individual silver NPs in glycerol solution onto a Formvar–carbon-coated 400 mesh copper TEM grid (Agar Scientific, UK) at RT until being dried and imaged on a FEI Tecnai TF20 FEG high-resolution TEM system, operating at 200 kV.

Techniques:

Journal: ACS Omega

Article Title: Novel Size-Tunable and Straightforward Ultra-Small Nanoparticle Synthesis in a Varying Concentration Range of Glycerol as a Green Reducing Solvent

doi: 10.1021/acsomega.3c02697

Figure Lengend Snippet: TEM/SEM micrograph images of silver NPs in glycerol (10–100%) and the corresponding size measurements. (a) 10% glycerol (Avg. 1.5 nm); (b) 20% glycerol (Avg. 37.22 nm); (c) 40% glycerol (Avg. 37.24 nm); (d) 60% glycerol (Avg. 36.44 nm); (e) 70% glycerol (Avg. 36.32 nm); (f) 80% glycerol (Avg. 37.24 nm); (g) 90% glycerol (Avg. 36.44 nm); and (h) 100% glycerol (Avg. 159.6 nm).

Article Snippet: The TEM samples were made ready by drop-casting 2 μL of individual silver NPs in glycerol solution onto a Formvar–carbon-coated 400 mesh copper TEM grid (Agar Scientific, UK) at RT until being dried and imaged on a FEI Tecnai TF20 FEG high-resolution TEM system, operating at 200 kV.

Techniques:

Journal: ACS Omega

Article Title: Novel Size-Tunable and Straightforward Ultra-Small Nanoparticle Synthesis in a Varying Concentration Range of Glycerol as a Green Reducing Solvent

doi: 10.1021/acsomega.3c02697

Figure Lengend Snippet: FTIR absorption spectrum of silver NPs in glycerol.

Article Snippet: The TEM samples were made ready by drop-casting 2 μL of individual silver NPs in glycerol solution onto a Formvar–carbon-coated 400 mesh copper TEM grid (Agar Scientific, UK) at RT until being dried and imaged on a FEI Tecnai TF20 FEG high-resolution TEM system, operating at 200 kV.

Techniques:

Journal: ACS Omega

Article Title: Novel Size-Tunable and Straightforward Ultra-Small Nanoparticle Synthesis in a Varying Concentration Range of Glycerol as a Green Reducing Solvent

doi: 10.1021/acsomega.3c02697

Figure Lengend Snippet: XRD patterns of the synthesized silver NPs in glycerol.

Article Snippet: The TEM samples were made ready by drop-casting 2 μL of individual silver NPs in glycerol solution onto a Formvar–carbon-coated 400 mesh copper TEM grid (Agar Scientific, UK) at RT until being dried and imaged on a FEI Tecnai TF20 FEG high-resolution TEM system, operating at 200 kV.

Techniques: Synthesized

Journal: ACS Omega

Article Title: Novel Size-Tunable and Straightforward Ultra-Small Nanoparticle Synthesis in a Varying Concentration Range of Glycerol as a Green Reducing Solvent

doi: 10.1021/acsomega.3c02697

Figure Lengend Snippet: AFM analysis of silver NPs in 100% glycerol to investigate the dispersion and aggregation of nanomaterials, in addition to their size, shape, absorption, and structure.

Article Snippet: The TEM samples were made ready by drop-casting 2 μL of individual silver NPs in glycerol solution onto a Formvar–carbon-coated 400 mesh copper TEM grid (Agar Scientific, UK) at RT until being dried and imaged on a FEI Tecnai TF20 FEG high-resolution TEM system, operating at 200 kV.

Techniques: